SRP6174

Ubiquitin-K0 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), 95% (HPLC)

• UNSPSC Code: 12352200

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE); 95% (HPLC)
• form: liquid
• mol wt: 8.760 kDa
• packaging: pkg of 500 μg
• technique(s): cell culture | mammalian: suitable
• NCBI accession no.: P62979
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RPS27A(6233)

Description

General description

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines. This molecule will not participate in polyubiquitin formation, and can therefore be used in control experiments for the study of ubiquitylation. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

Physical form

4 mg/mL in PBS.

Preparation Note

Centrifuge the vial prior to opening.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable