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SRP4835

Sigma-Aldrich

PCNA human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE), ≥90% (HPLC)

Synonym(s):

Cyclin, MGC8367, PCNA, Proliferating cell nuclear antigen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥90% (HPLC)
≥90% (SDS-PAGE)

form

liquid

mol wt

~29.8 kDa

packaging

pkg of 5 μg

impurities

endotoxin, tested

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PCNA(5111)

General description

PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein. PCNA is a homotrimeric protein known to localize in the nucleus. The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it.

Biochem/physiol Actions

Proliferating cell nuclear antigen (PCNA) has its main function in DNA replication and repair mechanism. Itsignificantly maintains the longevity of mature neutrophil granulocytes. Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma. PCNA supports cell survival by elevating metabolism flux. This gene expression is known to offer resistance to chemotherapy. Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

Physical form

Sterile filtered; 5 μg/5 μl in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junko Odajima et al.
PLoS genetics, 12(11), e1006429-e1006429 (2016-11-10)
E-type cyclins (cyclins E1 and E2) are components of the cell cycle machinery that has been conserved from yeast to humans. The major function of E-type cyclins is to drive cell division. It is unknown whether in addition to their
Cytoplasmic proliferating cell nuclear antigen connects glycolysis and cell survival in acute myeloid leukemia
Ohayon D, et al.
Scientific reports, 6, 35561-35561 (2016)
Proliferating cell nuclear antigen prevents trinucleotide repeat expansions by promoting repeat deletion and hairpin removal.
Beaver JM
DNA Repair, 48, 17-29 (2016)
Simran Kaushal et al.
Cell reports, 27(4), 1151-1164 (2019-04-25)
Common fragile sites (CFSs) are genomic regions that display gaps and breaks in human metaphase chromosomes under replication stress and are often deleted in cancer cells. We studied an ∼300-bp subregion (Flex1) of human CFS FRA16D in yeast and found that
Fahad R Ali et al.
Development (Cambridge, England), 141(11), 2216-2224 (2014-05-14)
Generation of neurons from patient fibroblasts using a combination of developmentally defined transcription factors has great potential in disease modelling, as well as ultimately for use in regeneration and repair. However, generation of physiologically mature neurons in vitro remains problematic.

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