SRP3141

Persephin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): PSP, PSPN
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 10.3 kDa
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: O60542
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PSPN(5623)

Description

General description

Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to glial cell line-derived neurotrophic factor (GDNF), artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFRa (a1-a4) receptors. The GFRa4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. The gene coding for persephin is localized on human chromosome 19p13.3. Recombinant human persephin is a disulfide-linked homodimer, composed of two 10.3kDa polypeptide chains (192 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Biochem/physiol Actions

Persephin stimulates the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve axotomy, and like GNDF, promotes ureteric bud branching. However, persephin does not support the survival of peripheral neurons.

Sequence

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG

Physical form

Lyophilized from 0.3x PBS, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable