SRP0238

cMet Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Synonym(s): HGFR, Hepatocyte growth factor receptor, RCCP2, Scatter factor receptor, met Proto-oncogene
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• mol wt: 74 kDa
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: X54559
• UniProt accession no.: P08581
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MET(4233)

Description

General description

Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.