SAB4200227

Anti-NOX1 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-GP91-2, Anti-MOX1, Anti-NADPH oxidase 1, Anti-NOH-1, Anti-NOH1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-10 μg/mL using using human colon; western blot: 1.5-3.0 μg/mL using using HT29 cell extracts
• UniProt accession no.: Q9Y5S8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NOX1(27035)

Description

General description

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1) belongs to the family of NADPH oxidases. It is abundantly expressed in colon, primarily in differentiated epithelial cells. NOX1 has two cytosolic regulators, NOXA1 and NOXO1 as well as Ras-related protein Rac1.

Immunogen

synthetic peptide corresponding to the N-terminal of human NOX1 isoform long (NOX1L). The corresponding sequence is identical in human NOX1 isoform long variant (NOX1LV) and highly conserved in mouse and rat NOX1 (76% sequence identity).

Application

Anti-NOX1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1) catalyzes the generation of superoxide ion. It is mitogenic and a potent trigger of the angiogenic switch, increasing the vascularity of tumors and inducing molecular markers of angiogenesis.. NOX1 promotes neurotoxic activation of microglia suggesting, that they play a central role during neuroinflammatory states and in amyotrophic lateral sclerosis (ALS).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable