S6402
Streptavidin−Cy3™ from Streptomyces avidinii
buffered aqueous solution
Synonym(s):
Streptavidin Cy3
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About This Item
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conjugate
CY3 conjugate
form
buffered aqueous solution
extent of labeling
3-9 mol fluorochrome per mol protein
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
General description
Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.
Application
Streptavidin−Cy3™ from Streptomyces avidinii has been used:
- in double immunofluorescence staining to detect biotinylated lectin
- for insulin and C-peptide staining in immunofluorescence analysis
- in double immunofluorescence to verify the presence of cells filled with biocytin
- in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
- with the traditional rhodamine filter arrangements due to the spectral overlap
Features and Benefits
Spectral Characteristics of Cy3 Dye:
- Absorbance Max 552 nm
- Emission Max 565 nm
- Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide
Legal Information
Cy3 is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Cell and tissue research, 332(3), 435-446 (2008-04-04)
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Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together
Articles
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