Proteins cannot be transferred to a membrane after being stained and de-stained with the Product RPROB, Reversible Protein Detection Kit.
RPROB
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.
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usage
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
Quality Level
technique(s)
protein staining: suitable
storage temp.
2-8°C
Application
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels[1] with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
Other Notes
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
Kit Components Only
Product No.
Description
- Solution A 500 mL
- Solution B 500 mL
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class Code
8B - Non-combustible corrosive hazardous materials
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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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When using Product RPROB, Reversible Protein Detection Kit, to stain protein on PAGE gels, can the protein be transferred from the gel to a membrane after destaining?
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