R4268

RNA Sample Loading Buffer

for NA electrophoresis, with ethidium bromide (50 μg/mL)

• MDL number: MFCD00213041
• UNSPSC Code: 12161703
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• form: liquid
• concentration: 1.25 ×
• technique(s): electrophoresis: suitable
• foreign activity: RNase, none detected
• storage temp.: −20°C; −20°C

Description

General description

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.

Application

RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.

Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.
RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.[1][2][3]

Components

Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.

RNA loading buffer contains 62.5% deionized formamide, 1.14M formaldehyde, 200 μg/ml bromphenol blue, 200 μg/ml xylene cyanole, and 50 μg/ml ehtidium bromide in MOPS-EDTA-sodium acetate at 1.25x working concentration.

Quantity

Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H332 - H341 - H350 - H360D - H373
• Precautionary Statements: P202 - P260 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Carc. 1B - Muta. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable