R4001
Ribonuclease A-agarose
ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)
Synonym(s):
Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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biological source
protein from bovine pancreas
Quality Level
material
beaded agarose
form
ammonium sulfate suspension
specific activity
400-1,000 units/g agarose (One ml gel will yield 12-30 units)
mol wt
~13,700
storage temp.
2-8°C
Physical form
Suspension in 2.0 M (NH4)2SO4 pH 7.0
Analysis Note
Electrophoretically and enzymatically assayed. RNase A and B contents determined by SDS-PAGE. Protein determined by E1%/280.
inhibitor
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Description
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A spectrophotometric method for the measurement of ribonuclease activity.
M KUNITZ
The Journal of biological chemistry, 164(2), 563-568 (1946-08-01)
Marka van Blitterswijk et al.
PloS one, 7(11), e48983-e48983 (2012-11-17)
Progressive muscular atrophy (PMA) and amyotrophic lateral sclerosis (ALS) are devastating motor neuron diseases (MNDs), which result in muscle weakness and/or spasticity. We compared mutation frequencies in genes known to be associated with MNDs between patients with apparently sporadic PMA
Yuen Lai Shek et al.
Biochemistry, 52(4), 672-680 (2013-01-09)
We report the first application of volume and compressibility measurements to characterization of interactions between cosolvents (osmolytes) and globular proteins. Specifically, we measure the partial molar volumes and adiabatic compressibilities of cytochrome c, ribonuclease A, lysozyme, and ovalbumin in aqueous
Chunju Li et al.
Journal of biotechnology, 162(2-3), 283-288 (2012-09-25)
Ribonuclease A (RNase A) is a therapeutic enzyme with cytotoxic action against tumor cells. Its clinical application is limited by the short half-life and insufficient stability. Conjugation of albumin can overcome the limitation, whereas dramatically decrease the enzymatic activity of
Adriano Chiò et al.
Neurology, 79(19), 1983-1989 (2012-10-27)
To assess the frequency and clinical characteristics of patients with mutations of major amyotrophic lateral sclerosis (ALS) genes in a prospectively ascertained, population-based epidemiologic series of cases. The study population includes all ALS cases diagnosed in Piemonte, Italy, from January
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