PURD10005
Pur-A-Lyzer™ Midi Dialysis Kit
Midi 1000, capacity 50-800 μL, MWCO 1 kDa
Synonym(s):
Dialyzers, High through put dialysis tubing, puralyzer
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About This Item
UNSPSC Code:
41123100
NACRES:
NB.22
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material
regenerated cellulose membrane
description
includes floating rack and handbook
packaging
pkg of 5 Maxi dialysis tubes
capacity
50-800 μL
tube L × diam.
5.2 cm × 1.8 cm
pore size
1 kDa MWCO
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General description
Pur-A-Lyzer dialysis tube kits are easy-to-use devices in a screw-cap tube format and are disposable. They contain a dual dialysis membrane window for buffer exchange. Samples are added and removed using a standard laboratory pipette and come in 4 different versions to accommodate a range of sample volumes.
- High sample recovery >97%
- Protease-, RNase-, and DNase-free
- Convenient sample loading- no syringes necessary
- Versatile- dialyze proteins, RNA, and DNA
The Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
Application
- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 μL
Midi: 50-800 μL
Maxi: 0.1-3 mL
Mega: 3-20 mL
Legal Information
Pur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLC
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Bhagyashree D Rao et al.
Chemistry and physics of lipids, 226, 104849-104849 (2019-12-15)
pH (low) insertion peptide (pHLIP) is a polypeptide from the third transmembrane helix of bacteriorhodopsin. The pH-dependent membrane insertion of pHLIP has been conveniently exploited for translocation of cargo molecules and as a novel imaging agent in cancer biology due
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