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P9792

Sigma-Aldrich

Phytase from Aspergillus ficuum

lyophilized powder, 1-5 units/mg solid (as phosphatase activity)

Synonym(s):

3-Phytase, myo-Inositol-hexakisphosphate 3-phosphohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

1-5 units/mg solid (as phosphatase activity)

storage temp.

−20°C

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Quality

May contain glucosidases and phosphatases.

Unit Definition

One unit will liberate 1.0 μmole of inorganic P from 4.2 × 10-2 M Mg·K phytate per min at pH 2.5 at 37 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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G C Irving et al.
Journal of bacteriology, 112(1), 434-438 (1972-10-01)
The fungus Aspergillus ficuum NRRL 3135 is known to produce an extracellular nonspecific orthophosphoric monoester phosphohydrolase (EC 3.1.3.2) with a pH optimum of 2.0, as well as an extracellular myo-inositol hexaphosphate phosphohydrolase (EC 3.1.3.8; phytase) with pH optima of 2.0
Ion-exchange chromatography of inositol polyphosphates.
D J Cosgrove
Annals of the New York Academy of Sciences, 165(2), 677-686 (1969-10-17)
L Jermutus et al.
Journal of biotechnology, 85(1), 15-24 (2001-02-13)
Thermostability is a key feature for commercially attractive variants of the fungal enzyme phytase. In an initial set of experiments, we restored ionic interactions and hydrogen bonds on the surface of Aspergillus terreus phytase, which are present in the homologous
Nada M Tamim et al.
Journal of agricultural and food chemistry, 51(16), 4687-4693 (2004-01-07)
Phytate phosphorus (PP) hydrolysis by a 3-phytase was studied in vitro at pH 2.5 and 6.5 with either 0, 1.0, 4.0, or 9.0 g of Ca/kg diet, or 0, 1.0, 5.0, 7.5, or 10.0 g/kg diet of micro-mineral premix added
Duane F Berry et al.
Bioorganic & medicinal chemistry letters, 15(12), 3157-3161 (2005-05-10)
A novel approach for measuring phytase activity is presented. We have developed a new chromophoric substrate analog of phytic acid, 5-O-[6-(benzoylamino)hexyl]-d-myo-inositol-1,2,3,4,6-pentakisphosphate that permits direct measurement of the phosphate ester bond-cleavage reaction using HPLC. This compound, along with its dephosphorylated T-phosphatidylinositol

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