The concentration can be confirmed on the Certificate of Analysis that's specific to the lot you received, found here:https://www.sigmaaldrich.com/US/en/product/sigma/p2980#product-documentation
As of April 2024, the current specification is 0.2mg/mL
P2980
Pertussis toxin
Pertussis toxin from Bordetella pertussis
from Bordetella pertussis, buffered aqueous glycerol solution, protein endotoxin
Synonym(s):
Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
Recommended Products
Product Name
Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution
description
aseptically packaged
Quality Level
form
buffered aqueous glycerol solution
shipped in
dry ice
storage temp.
−20°C
SMILES string
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
InChI
1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3
InChI key
WOPKHAQDUMDJIY-UHFFFAOYSA-N
Gene Information
Bordetella pertussis Tohama I ... ptxA(2665068) , ptxB(2665069) , ptxC(2665408)
Looking for similar products? Visit Product Comparison Guide
General description
Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP.
Application
Inactive toxin form released from B. pertussis.
Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis[1]; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model;[2] as an inhibitor of Gαi signaling[3]
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Biochem/physiol Actions
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Antonietta Franco et al.
Journal of molecular and cellular cardiology, 121, 60-68 (2018-07-04)
G-protein receptor kinases (GRKs) regulate adult hearts by modulating inotropic, chronotropic and hypertrophic signaling of 7-transmembrane spanning neurohormone receptors. GRK-mediated desensitization and downregulation of β-adrenergic receptors has been implicated in adult heart failure; GRKs are therefore a promising therapeutic target.
Jonathan Hoggatt et al.
Cell, 172(1-2), 191-204 (2017-12-12)
Hematopoietic stem cell transplantation is a potential curative therapy for malignant and nonmalignant diseases. Improving the efficiency of stem cell collection and the quality of the cells acquired can broaden the donor pool and improve patient outcomes. We developed a
Kevin C Kemp et al.
Acta neuropathologica, 135(6), 907-921 (2018-03-16)
Bone marrow-derived cells are known to infiltrate the adult brain and fuse with cerebellar Purkinje cells. Histological observations that such heterotypic cell fusion events are substantially more frequent following cerebellar injury suggest they could have a role in the protection
Jason M Conley et al.
The Journal of biological chemistry, 297(1), 100881-100881 (2021-06-19)
GPR17 is a G-protein-coupled receptor (GPCR) implicated in the regulation of glucose metabolism and energy homeostasis. Such evidence is primarily drawn from mouse knockout studies and suggests GPR17 as a potential novel therapeutic target for the treatment of metabolic diseases.
Jianghong Zhong et al.
Journal of neuroinflammation, 17(1), 113-113 (2020-04-12)
Increasing evidence has suggested that a single nucleotide polymorphism in the Ncf1 gene is associated with experimental autoimmune encephalomyelitis (EAE). However, the mechanisms of NCF1-induced immunoregulatory effects remain poorly understood. In this study, we focus on NCF1 deficiency-mediated effects on
Articles
Cyclic nucleotides like cAMP modulate cell function via PKA activation and ion channels.
-
We purchased the 50 uG size of the P2980 Pertussis Toxin. I am unable to find what the volume of solution in the container is to calculate uG/mL concentration.
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
