NPT01
NeuroPorter™ Transfection Kit
Lipid formulation for nucleic acid transfections in neuronal and glial cells
Synonym(s):
NeuroPorter Transfection, Transfection Kit
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.25
Recommended Products
grade
for molecular biology
Quality Level
form
dried film
usage
kit sufficient for 75-200 transfections
availability
available only in USA, Canada and EU
technique(s)
transfection: suitable
storage temp.
2-8°C
General description
Neuroporter™ Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.
Application
Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 μl Neuroporter™ Transfection Reagent and 6-8 μg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:
- C6 glioma (human)
- Cortical neurons (rat primary)
- Dorsal Root Ganglion (DRG) cells (rat)
- NT2 neurons(human precursor cells)
- NT neurons (human differentiated cells)
- Subventricular Zone (SVZ) cells (mouse)
- White matter cells (mouse)
Features and Benefits
- Optimized for primary neurons, glial cells, and cultured neural cell lines
- Very low toxicity with no neuro-degeneration or dendrite withdrawal
- Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
- Fast and easy to use compared to other methods
- Compatible with both serum and serum-free transfection protocols
Components
1 vial Neuroporter™ Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941
Caution
Do not freeze.
Principle
A stable, non-covalent complex is formed when the Neuroporter™ Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
Legal Information
NeuroPorter is a trademark of Gene Therapy Systems, Inc.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Adan Aguirre et al.
Nature, 467(7313), 323-327 (2010-09-17)
Specialized cellular microenvironments, or 'niches', modulate stem cell properties, including cell number, self-renewal and fate decisions. In the adult brain, niches that maintain a source of neural stem cells (NSCs) and neural progenitor cells (NPCs) are the subventricular zone (SVZ)
Beata Jablonska et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(42), 14775-14793 (2012-10-19)
Diffuse white matter injury (DWMI) caused by hypoxia is associated with permanent neurodevelopmental disabilities in preterm infants. The cellular and molecular mechanisms producing DWMI are poorly defined. Using a mouse model of neonatal hypoxia, we demonstrate a biphasic effect on
Beata Jablonska et al.
The Journal of cell biology, 179(6), 1231-1245 (2007-12-19)
We investigated the function of cyclin-dependent kinase 2 (Cdk2) in neural progenitor cells during postnatal development. Chondroitin sulfate proteoglycan (NG2)-expressing progenitor cells of the subventricular zone (SVZ) show no significant difference in density and proliferation between Cdk2(-/-) and wild-type mice
Nikhil G Thaker et al.
Journal of neuroscience methods, 185(2), 204-212 (2009-09-29)
A major challenge for the treatment of cancers, such as glioblastoma multiforme (GBM), has been resistance to radiation and cancer chemotherapeutics. Short interfering RNA (siRNA) based screening may facilitate the identification of genes and pathways essential for cancer cell survival
Simone Di Giovanni et al.
The Journal of biological chemistry, 280(3), 2084-2091 (2004-11-04)
Following spinal cord injury, there are numerous changes in gene expression that appear to contribute to either neurodegeneration or reparative processes. We utilized high density oligonucleotide microarrays to examine temporal gene profile changes after spinal cord injury in rats with
Articles
Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
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