N6009

Nerve Growth Factor-2.5S from murine submaxillary gland

NGF-2.5S, lyophilized powder, suitable for cell culture

• Synonym(s): NGF-2.5S
• CAS Number: 93928-24-6
• MDL number: MFCD00161772
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: mouse
• Quality Level: 200
• form: lyophilized powder
• potency: 0.1-30 ng/mL EC₅₀ (using PC-12 cells)
• mol wt: 26.5 kDa
• packaging: pkg of 4X25 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P01139
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Ngf(18049)

Description

General description

Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.

Application

Nerve Growth Factor-2.5S from murine submaxillary gland has been used:

• in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
• as a media supplement in the “process compartment” for microfluidic chamber studies
• to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression

Biochem/physiol Actions

Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.

Other Notes

2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves