MAK453

Glutathione S-transferase Assay Kit

Sufficient for 100 colorimetric tests

• Synonym(s): GST Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• input: cell lysate; tissue
• detection method: colorimetric
• relevant disease(s): neurological disorders, asthma; pulmonary disorders, diabetes; cancer; endocrinological disorders
• storage temp.: −20°C

Description

General description

Glutathione S-transferase (GST) is a vital enzyme involved in detoxification, aiding in the removal of harmful substances from cells. It plays a key role in metabolizing a wide range of compounds, including drugs, toxins, and carcinogens, and contributes to cellular processes such as signaling and inflammation regulation. Monitoring GST levels is crucial for several reasons: it serves as a biomarker for cellular stress, indicating damage from toxins or oxidative stress; it aids in diagnosing and managing various diseases, including cancer and liver diseases; it informs pharmacological studies by assessing drug efficacy and toxicity; and it facilitates environmental monitoring, identifying pollution and potential health risks. Overall, monitoring GST levels provides valuable insights into cellular health, disease status, drug metabolism, and environmental exposure, guiding early detection and management of health conditions.

Application

The Glutathione S-transferase Assay Kit may be used for:

• Cancer Research
• Oxidative Stress Research
• Metabolism Research

Features and Benefits

• Efficient and Precise: Requires only 20 μL of sample and a quick 10-minute reaction time while detecting Glutathione S-transferase levels within a range of 2 to 80 U/L, offering convenience and sensitivity.
• Compatibility with High-Throughput Systems: Easily integrate our kit into high-throughput handling systems, ensuring smooth operation and accurate results, enhancing efficiency in your laboratory processes.

Suitability

The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc.

Principle

The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at A₃₄₀ nm. The rate of increase in absorbance at A₃₄₀ nm is directly proportional to the GST activity in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H319,H412
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C