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Key Documents

MAK432

Sigma-Aldrich

Histamine Quantification Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Histamine Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

Quality Level

application(s)

food and beverages

detection method

colorimetric

storage temp.

−20°C

General description

Histamine is a biogenic amine, generated from the single decarboxylation of the amino acid histidine.[1] Histamine food poisonings are allergy-like food poisonings caused by the ingestion of spoiled food containing markedly elevated histamine levels.[2] Elevated levels of bacterial fermentation can lead to elevated histamine in various food and drink sources such as fish, fermented meat, milk, cheese, beer, and wine.[2]

Application

The Histamine Quantification Assay Kit may be used for:
  • Food and Beverage Testing
  • Bacterial Fermentation Analysis

Features and Benefits

Suitability

The kit is suitable for the quantification of histamine in various food and beverage samples.

Principle

The kit is based on the enzymatic oxidation of histamine, which is coupled to the reduction of the formazan WST reagent. The intensity of the product color, measured at 450 nm, is directly proportional to histamine concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids


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Evidence that histamine is the causative toxin of scombroid-fish poisoning.
Morrow J D, et al.
The New England Journal of Medicine, 324(11), 716-720 (1991)
Biomedical Aspects of Histamine
Khardori N, et al.
SpringerPlus (2011)

Questions

1–3 of 3 Questions  
  1. Will this kit give matric effect with other biogenic amines like Putrescine, cadaverine?

    1 answer
    1. Unfortunately, the matrix effect with other biogenic amines is not investigated for this kit.

      Helpful?

  2. What is the recommend type plate for use with the MAK432-1KT Histamine Quantification Assay Kit? is it ok to use a Nunc Maxisorp plate.

    1 answer
    1. QC testing for the kit recommends the use of M2936-100EA Greiner 96 well plates, polystyrene.

      Helpful?

  3. How long is the assay buffer in the MAK432-1KT Histamine Quantification Assay Kit stable for when stored at 2-8 degrees? The technical bulletin indicates that all components are good for 2 years at -20 degrees, but please confirm the stability period for the assay buffer once it's been thawed and stored at 4 degrees.

    1 answer
    1. The Assay Buffer remains stable at 2-8 degrees Celsius at least until the expiration date of the kit.

      Helpful?

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