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MAK077

Sigma-Aldrich

Uric Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; rheumatological diseases; genitourinary disorders

storage temp.

−20°C

General description

In humans and primates, uric acid is the end product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and excreted in urine. High serum levels of uric acid, hyperuricemia, are associated with insulin resistance, cardiovascular disease, and gout. The mechanisms leading to hyperuricemia are typically either increased uric acid production or decreased urine excretion. Increased serum uric acid may be a marker of renal disease.

Application

Uric Acid Assay Kit has been used
  • to determine the uric acid content in human adipose-derived stem cell culture supernatant
  • to measure serum uric acid level in order to analyse kidney function in rat models
  • to determine the uric acid concentration in serum and to study the e antiinflammatory effects of sunflower head ethanol extract in rats
  • in quantitative analysis of uric acid degradation

Suitability

Suitable for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required

Principle

In this assay, Uric Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the Uric Acid present.

related product

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Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Role for Uric Acid in the Progression of Renal Disease.
Kang, D.-H
Journal of the American Society of Nephrology, 12, 2888-2897 (2002)
Stephanie A Terry et al.
Frontiers in veterinary science, 6, 308-308 (2019-10-15)
The objective of this study was to examine the effect of a pine enhanced biochar (EB) on rumen fermentation, apparent total tract digestibility, methane (CH4) emissions, and the rumen and fecal microbiome of Angus × Hereford heifers fed a barley
Gabriel O Ribeiro et al.
Scientific reports, 7(1), 1276-1276 (2017-04-30)
Future growth in demand for meat and milk, and the socioeconomic and environmental challenges that farmers face, represent a "grand challenge for humanity". Improving the digestibility of crop residues such as straw could enhance the sustainability of ruminant production systems.
Stephanie A Terry et al.
Journal of animal science, 96(9), 3863-3877 (2018-09-01)
Ruminants play an important role in food security, but there is a growing concern about the impact of cattle on the environment, particularly regarding greenhouse gas emissions. The objective of this study was to examine the effect of humic substances
Effect of humic substances on rumen fermentation, nutrient digestibility, methane emissions, and rumen microbiota in beef heifers.
Terry S A, et al.
Journal of Animal Science (2018)

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