MAK020
Fructose-6-Phosphate Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Fructose-6-P Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Fructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.
Application
Fructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).
Suitability
Suitable for quantifying Fructose-6-Phosphate in a variety of samples
Principle
Fructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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