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MAK002

Sigma-Aldrich

L-Amino Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

L-Amino Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new L-Amino Acid Assay Kit, MAK490, is now available!

L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules. While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.

Suitability

Suitable for quantitating L-amino acids in body fluids or purified samples.

Principle

In this assay, L-Amino Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-Amino Acids present. Glycine is not detectable by this assay.

replaced by

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Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analysis of endogenous D-amino acid-containing peptides in metazoa.
Bai L, et al.
Bioanalytical reviews., 1(1), 7-24 (2009)
Jinrang Kim et al.
Endocrinology, 160(5), 979-988 (2019-04-03)
Plasma amino acids and their transporters constitute an important part of the feedback loop between the liver and pancreatic α-cell function, and glucagon regulates hepatic amino acid turnover. Disruption of hepatic glucagon receptor action activates the loop and results in
Peng Shang et al.
Aging cell, 16(2), 349-359 (2017-01-14)
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Alberts B, et al.
Molecular biology of the Cell. 4th Edition. (2002)
Alberts B, et al.
Molecular Biology in Plant Pathogenesis and Disease Management (2002)
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