K3753
Krebs-Henseleit Buffer Modified
With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
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About This Item
UNSPSC Code:
12352207
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General description
Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.
Application
Krebs-Henseleit Buffer Modified has been used:
- to collect the rat brains for ex vivo brain slice experiments
- to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
- to collect the uterine and forelimb blood vessel samples from horses
Quantity
Formulated to contain 9.6 grams of powder per liter of medium.
Reconstitution
Supplement with 2.1 g/L sodium bicarbonate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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