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H6041

Sigma-Aldrich

Interferon α 2A human

IFN-α 2A, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym(s):

α 2A Interferon, Human Interferon α 2A, IFN-α 2A

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.4 ng/mL EC50

quality

endotoxin tested

mol wt

monomer 16 kDa (glycosylated)

packaging

glass bottle of 10 μg (in glass bottle)

manufacturer/tradename

HumanZyme

technique(s)

cell culture | mammalian: suitable

impurities

≤1EU/mg

UniProt accession no.

P01563

storage temp.

−20°C

Gene Information

human ... IFNA2(3440)

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General description

The IFNA2 (interferon alpha 2) gene is mapped to human chromosome 9p21.3. It is considered to be a prototypic type I IFN subtype. The encoded protein contains 165 amino acid. Ifna2 protein structure possesses five α-helices, linked by two disulfide bonds.

Application

Interferon α 2A human has been used to check its antiviral action against chikungunya virus. It has also been used as a control to check the antiviral cytokine induction of Korean red ginseng.

Biochem/physiol Actions

IFNA2 is a type 1 interferon which acts as the first line of defense, and offers protection against pathogenic invasion.
Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. It has antiproliferative and antitumor activities, as well as induction of apoptosis and the inflammatory response. IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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IFNA2: The prototypic human alpha interferon
Paul F, et al.
Gene, 567(2), 132-137 (2015)
Loss of tumor suppressive microRNA-31 enhances TRADD/NF-kappaB signaling in glioblastoma
Rajbhandari R, et al.
Testing, 6(19), 17805-17805 (2015)
Evaluation of broad-spectrum antiviral compounds against chikungunya infection using a phenotypic screening strategy
Bonotto RM, et al.
F1000Research, 7 (2018)
A R Mire-Sluis et al.
Journal of immunological methods, 195(1-2), 55-61 (1996-09-09)
Interferons-alpha and -beta (IFN-alpha and -beta) are cytokines that are widely known to induce potent anti-viral activity. However, it has become increasingly apparent that IFN-alpha and -beta exert a variety of other biological effects, including anti-tumour and immunomodulatory activities and
Gillian M Keating
Drugs, 69(18), 2633-2660 (2009-12-01)
Peginterferon-alpha-2a (40 kD) [Pegasys] comprises an inert, branched, 40 kD polyethylene glycol (PEG) moiety attached to interferon-alpha-2a. Subcutaneous peginterferon-alpha-2a (40 kD) is indicated for the treatment of adults with hepatitis B e antigen (HBeAg)-positive or -negative chronic hepatitis B who
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