F2016
Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
rabbit
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
indirect immunofluorescence: 1:160 using mouse splenocytes
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Binds all goat Igs.
IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
Application
Anti-Goat IgG (whole molecule)-FITC antibody produced in rabbit has been used in immunofluorescence to study the translocation of CXCR4 in human hepatoma cell lines. It has also been used in immunocytochemistry.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Goat IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.
Other Notes
Antibody adsorbed with human serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Jeremiah Morrissey et al.
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