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DUO87007

Sigma-Aldrich

Duolink® PLA® Probe Human IgG Isotype Control PLUS human

suitable for proximity ligation assay

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.41

biological source

human serum (polyclonal)

Quality Level

200

antibody form

affinity purified immunoglobulin (Isotype control antibody)

product line

Duolink®

technique(s)

proximity ligation assay: suitable

suitability

suitable for brightfield
suitable for fluorescence

shipped in

wet ice

storage temp.

2-8°C

General description

An immunoglobulin G (IgG) isotype control antibody with the Duolink® PLA® PLUS oligonucleotide specific for human IgG. This is a convenience product to be used in place of a human primary antibody to determine non-specific background signal and optimal antibody dilution. The anti-human PLA® probe PLUS is not necessary when using this product, but must be used in combination with a MINUS PLA® probe.

Application

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
Application Note

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Isotype control PLA probes are used in lieu of a primary antibody in a Duolink® PLA in situ experiment to determine background signal contributed by non-specific binding of the primary antibody. Isotype control PLA probes can also be used to determine the optimal titer conditions of primary antibody PLA probes.

A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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