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D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonym(s):

DNA single stranded

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About This Item

CAS Number:
91080-16-9
EC Number:
293-507-3
MDL number:
MFCD00163436
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

form

lyophilized powder

mol wt

~50 kb

composition

ssDNA, ≥65%

storage temp.

−20°C

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General description

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay[1] and in DNA-binding assay.[2]
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantity

One mg DNA is approx. 25 A260 units.

Preparation Note

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.[3]

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000337348
0000337348
SLCC4481
SLBX9106
SLBV8052

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H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
Qiong Guo et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 106, 155-162 (2013-02-05)
A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff
Eswaran Ramachandran et al.
Inorganic chemistry, 52(3), 1504-1514 (2013-01-18)
A series of four new palladium(II) complexes of 2-oxo-1,2-dihydroquinoline-3-carbaldehyde thiosemicarbazones with triphenylphosphine as coligand have been synthesized and characterized by the aid of various spectral techniques. The single-crystal X-ray diffraction studies revealed that the unsubstituted thiosemicarbazone ligand acted as monobasic
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
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Questions

  1. Does the product reanneal after storage and cooling at room temperature? Should we boil the ssDNA prior to use? How many base pairs are included in this ssDNA?

    1 answer

    1. Unfortunately, information regarding the reanneal after storing and cooling at room temperature has not been determined.

      Product D8899 is listed as having a length of approximately 50 kb. For single stranded DNA, the molecular weight of one kb is approximately equal to 3.3 × 105 daltons, so 50 kb has a weight of approximately 16.5 × 106 daltons.

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