CS0001
Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)
sufficient for 200 colorimetric or fluorometric tests
Synonym(s):
DDPC, DEPC, DLOPC, DLPC, DMPC, DOPC, DPPC, DSPC, EPC, HEPC, HSPC, LYSOPC, MPPC, MSPC, PC, PMPC, POPC, PSPC, Phosphatidylcholine, SMPC, SOPC, lecithin, lysolecithin, sphingomyelin
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.84
Recommended Products
usage
sufficient for 200 colorimetric or fluorometric tests
shipped in
dry ice
storage temp.
−20°C
General description
Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline.
The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).
The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).
Application
- Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
- Enables evaluation of drug effects on phospholipids metabolism
Features and Benefits
Our economical Phospholipid quantification assay kit also saves your time and efforts:
- Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples
- User friendly: No need to weigh or calculate needed amounts of reagents on your own
- Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains sufficient reagents for 200 tests.
- Suitable for both colorimetric and fluorometric detection
- Detection time: only 30 minutes
Suitability
Suitable for the detection of phospholipids in biological samples
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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