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CG201

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit

sufficient for 96 assays

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

usage

sufficient for 96 assays

Quality Level

200

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592)

General description

cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The immunoassay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

Application

Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. It has also been used to assess the guanylate cyclase activity.

Kit Components Only

Product No.
Description
  • Acetic Anhydride 1 x 1
  • Assay Buffer 2 1 x 30
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP Assay Layout Sheet 1 x 1
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • 5 Cycle Logit-Log Paper 1 x 1
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
See All (13)

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

12.2 °F - closed cup

Flash Point(C)

-11 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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