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biological source
Mouse x Mouse Hybridoma
ImmunoDon
Balb/c mouse spleen
Myeloma
NS0 mouse myeloma
Reactivity
Rat IgG2b heavy chain
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
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Cell Line Origin
Monoclonal antibody rat IgG2b
Cell Line Description
Mouse hybridoma against rat IgG2b heavy chain. Cambridge Enterprise Reference number AB71:www.enterprise.cam.ac.uk.
Immunogen
Rat IgG
Culture Medium
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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