A8887

Acrylamide

suitable for electrophoresis, ≥99%

• Synonym(s): 2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
• Linear Formula: CH₂=CHCONH₂
• CAS Number: 79-06-1
• Molecular Weight: 71.08
• Beilstein: 605349
• EC Number: 201-173-7
• MDL number: MFCD00008032
• UNSPSC Code: 41105319
• eCl@ss: 39031205
• PubChem Substance ID: 24891378
• NACRES: NB.22

Properties

• vapor density: 2.45 (vs air)
• Quality Level: 200
• vapor pressure: 0.03 mmHg ( 40 °C)
• Assay: ≥99%
• form: crystals
• technique(s): electrophoresis: suitable
• bp: 125 °C/25 mmHg (lit.)
• mp: 82-86 °C (lit.)
• solubility: water: soluble 2.5 g/10 mL, clear, colorless
• suitability: suitable for electrophoresis
• SMILES string: NC(=O)C=C
• InChI: 1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
• InChI key: HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

General description

Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide has been used:

• for the synthesis of hydrogels
• for the preparation of polyacrylamide gel
• to agglomerate vimentin and keratin intermediate filament network

Acrylamide has been used:

• to agglomerate the vimentin and keratin intermediate filament network of K562cells
• in the preparation and characterization of soft culture substrate
• in hydrogel synthesis
• for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301,H312 + H332,H315,H317,H319,H340,H350,H361f,H372
• Precautionary Statements: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 280.4 °F - closed cup
• Flash Point(C): 138 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves