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A8412

Sigma-Aldrich

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75
Pricing and availability is not currently available.

biological source

bovine

Quality Level

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

Assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative)
cell growth >50%

shipped in

ambient

storage temp.

2-8°C

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General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Pinar Sahin et al.
Fertility and sterility, 102(5), 1482-1490 (2014-09-23)
To evaluate the expression of mammalian target of rapamycin (mTOR) pathway molecules in mouse spermatogenesis and as well as its role during proliferation and meiotic initiation of spermatogenic cells. Experimental animal study. University. C57Balb-C adult male mice. Expressions of mTOR
Amanda J Shapiro et al.
Veterinary microbiology, 177(1-2), 154-161 (2015-03-18)
The seroprevalence of Coxiella burnetii (C. burnetii) in cats in eastern Australia is unknown, and the risk of transmission from cats to humans is undetermined. This study aimed to determine the exposure of cats to C. burnetii in four distinct
Eric M Kernfeld et al.
Immunity, 48(6), 1258-1270 (2018-06-10)
Thymus development is critical to the adaptive immune system, yet a comprehensive transcriptional framework capturing thymus organogenesis at single-cell resolution is still needed. We applied single-cell RNA sequencing (RNA-seq) to capture 8 days of thymus development, perturbations of T cell receptor rearrangement
Paul J Orchard et al.
Scientific reports, 9(1), 7858-7858 (2019-05-28)
Cerebral adrenoleukodystrophy (cALD) is an inflammatory neurodegenerative disease associated with mutation of the ABCD1 gene. Proteomic analysis of cerebral spinal fluid (CSF) from young males with active cALD revealed markers of inflammation including APOE4. APOE4 genotype has been associated with
Katherine Shortt et al.
Scientific reports, 9(1), 1396-1396 (2019-02-06)
Acetaminophen (APAP) is a commonly used analgesic responsible for more than half of acute liver failure cases. Identification of previously unknown genetic risk factors would provide mechanistic insights and novel therapeutic targets for APAP-induced liver injury. This study used a

Articles

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Questions

1–2 of 2 Questions  
  1. Is this product fatty acid free?

    1 answer
    1. This product is not fatty acid free, it is fatty-acid depleted. The product is not tested for free fatty acid content. Please see several options below for fatty-acid free BSAs offered:
      https://www.sigmaaldrich.com/product/sigma/a8806
      https://www.sigmaaldrich.com/product/sigma/a7030
      https://www.sigmaaldrich.com/product/sigma/a1595
      https://www.sigmaaldrich.com/product/mm/126609

      Helpful?

  2. La référence A8412 a t-elle une date de retest ou d'expiration donnée par le fournisseur svp ?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

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