Skip to Content
Merck
All Photos(1)

Documents

72091

Sigma-Aldrich

NBT-BCIP® solution

BioReagent, suitable as substrate for alkaline phosphatase in dot blots

Synonym(s):

BCIP®/NBT, NBT-X-phosphate

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
329763805
NACRES:
NA.21

grade

suitable as substrate for alkaline phosphatase in dot blots

product line

BioReagent

form

liquid

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChI key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

152.6 °F - closed cup

Flash Point(C)

67 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

D Thompson et al.
BioTechniques, 12(5), 656-658 (1992-05-01)
A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels
Andrea L H Arnett et al.
Frontiers in microbiology, 2, 220-220 (2011-11-09)
Recombinant adeno-associated viral (rAAV) vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for
Dariusz Abramczyk et al.
Preparative biochemistry & biotechnology, 39(3), 277-288 (2009-05-12)
The class V chitin synthase is unique because it has a myosin motor-like domain fused to its catalytic domain. The biochemical properties of this enzyme and its function remain undefined beyond the knowledge that it is the only single chitin
Sandra A Calarota et al.
Vaccine, 26(40), 5188-5195 (2008-05-09)
IL-7 and IL-15 are key cytokines involved in the generation and maintenance of memory CD8+ T-cells. We evaluated these cytokines as molecular adjuvants for topical HIV-1 DermaVir vaccine. We found that mice receiving DermaVir formulated with HIV-1 Gag plasmid in
Hyun-Jung Kim et al.
The International journal of developmental biology, 53(7), 993-1002 (2009-07-15)
The current-producing cells of the electric organ, i.e., electrocytes, in Sternopygus macrurus derive from skeletal muscle fibers. Mature electrocytes are not contractile, but they do retain some muscle proteins, are multinucleated, and receive cholinergic innervation. Electrocytes express the myogenic regulatory
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service