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N4638

Sigma-Aldrich

Neutral Red

BioReagent, Powder

Synonym(s):

3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
553-24-2
Molecular Weight:
288.78
Colour Index Number:
50040
Beilstein:
3918740
EC Number:
209-035-8
MDL number:
MFCD00012651
UNSPSC Code:
12171500
PubChem Substance ID:
24897707
NACRES:
NA.47

product name

Neutral Red, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

cell culture | mammalian: suitable
titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:
  • as a nuclear stain
  • as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
  • fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
  • detection of viable yeast cells in dandruff samples and of mussel larvae
  • Neutral red has also been used as a stain in flow cytometry.
Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000370047
0000343198
0000366296
SHBL2316
MKCG8905

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