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75405

Millipore

Orange-serum Agar

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17 g/L
casein peptone, 10 g/L
dipotassium hydrogen phosphate, 3 g/L
D(+)-glucose, 4 g/L
orange extract, 5 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
microbiology

suitability

selective by low pH for Aspergillus spp.
selective by low pH for Bacillus spp.
selective by low pH for Candida spp.
selective by low pH for Lactobacillus spp.
selective by low pH for Penicillium spp.
selective by low pH for Saccharomyces spp.
selective by low pH for aciduric bacteria
selective by low pH for bacteria (General Media)
selective by low pH for molds (General Media)
selective by low pH for yeasts (General Media)

Application

Orange-serum Agar can be used for the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit, according to Hays, Troy and Beisel.
The prepared agar may be turbid after autoclaving due to orange extract.
Orange-serum Agar has been used to prepare modified orange serum (MOS) as a medium for Hymenoscyphus fraxineus.

Preparation Note

Dissolve 42 g in 1 litre distilled water. Autoclave gently at 115°C for 15 minutes taking care not to overheat. The prepared agar may be turbid after autoclaving due to orange extract.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL9748
BCCK6409
BCCJ6638
BCCF2074
BCCB2232

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D.I. Murdock et al.
Food Technology, 6, 181-181 (1952)
G.L. Hays et al.
Food Technology, 6, 386-386 (1952)
ADA, a fast-growth medium for Hymenoscyphus fraxineus
L. Botella
Forest Pathology, 46 (2016)

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