This dye can be helpful in visualizing the microtubules of a cell. If you are studying a cancer cell line, then the natural dynamics of the microtubules will occur as the cells go through their cycle and divide. It's worth considering whether you want to add an agent to induce mitosis further, especially as you are trying to see the effects of a drug on this mechanism as it occurs.
SCT142
BioTracker 488 Green Microtubule Cytoskeleton Dye
Live cell imaging microtubule dye for imaging the cytoskeleton of living cells
Synonym(s):
Live cell imaging probe
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
Pricing and availability is not currently available.
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technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
General description
Cytoskeleton proteins such as actin and tubulin play an important role in cell structure and motility. Dysregulation of cytoskeletal proteins are also involved in cancer metastasis and disease. Taxol binds to polymerized tubulin and stabilizes microtubules, resulting in inhibition of mitosis. However, fluorescent taxol compounds like the BioTracker Microtubule Cytoskeleton dyes are less disruptive of microtubule dynamics and cell division, presumably due to lower binding affinity of the fluorescent probe compared to taxol itself.
The BioTracker Cytoskeleton Microtubule Dye is a green fluorescent cell-permeable taxol probe for imaging the microtubule cytoskeleton in live cells. It is a simple, rapid and sensitive stain that can be detected using the FITC channel. The dye is supplied with a vial of 100mM verapamil, an efflux pump inhibitor that may improve probe retention and staining in certain cell types.
Spectral Properties
Absorbance: 500nm
Emission: 515nm
The BioTracker Cytoskeleton Microtubule Dye is a green fluorescent cell-permeable taxol probe for imaging the microtubule cytoskeleton in live cells. It is a simple, rapid and sensitive stain that can be detected using the FITC channel. The dye is supplied with a vial of 100mM verapamil, an efflux pump inhibitor that may improve probe retention and staining in certain cell types.
Spectral Properties
Absorbance: 500nm
Emission: 515nm
Application
Live cell fluorescent imaging
Live cell imaging microtubule dye for imaging the cytoskeleton of living cells
Research Category
Cell Signaling
Cell Signaling
Research Sub Category
Live Cell Dye
Live Cell Dye
Components
1) 1 vial of 50µL BioTracker 488 Green Microtubule Cytoskeleton Dye (1000X in DMSO) (CS224593)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
Quality
Spectral Properties
Absorbance: 500nm
Emission: 515nm
Absorbance: 500nm
Emission: 515nm
Physical form
Liquid
Storage and Stability
Store BioTracker 488 Green Microtubule Cytoskeleton Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Would it be advisable to employ any form of stimulus to induce microtubular organization in cancer cells when treating them with our drug to inhibit migration and analyze cytoskeletal changes, given that we have acquired the green microtubule dye?
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