MEKK5 (MAP3K5) is a key kinase for our innate immunity and for mediating cellular signaling cascades evoked by changes in the external cellular environment. As such, MEKK5 is critical for cell fate during development, apoptosis, and cell survival as well as in modulating the host defense responses from a wide range of pathogens. MEKK5 acts as an upstream activator of the MKK/JNK signaling pathways and the p38 MAPK signaling pathways that ultimately results in activating multiple kinases such as MAP2K3/Mkk3 or MKK6/MMK7. Activation of MMEKK5 is via phosphorylation of Thr-845 within the activation loop via the recently discovered kinase MAP3K6 which forms a complex with MEKK5 and also stabilizes the protein. Expression is widespread and localized in the cytoplasmic in the perinuclear endoplasmic reticulum region.
Immunogen
linear peptide corresponding to Human phospho MEKK5 (Thr 845).
Application
Anti-phospho MEKK5, (Thr845), clone PA214 | MABS494 is an antibody against phospho MEKK5 for use in Western Blotting.
Research Category Signaling
Research Sub Category Immunological Signaling
Quality
Evaluated by Western Blotting on HEK293A lysate Western Blotting Analysis: 4 µg/mL dilution of this antibody detected phosphorylated MEKK5 protein in 10 µg of H2O2 treated Hek293A cell lysate
Target description
~160 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rabbit monoclonal supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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