MABE429
Anti-UBE2I Antibody, clone 1B10
ascites fluid, clone 1B10, from mouse
Synonym(s):
SUMO-conjugating enzyme UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1B10, monoclonal
species reactivity
monkey, human
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UBE2I(7329)
General description
The protein encoded by the human gene UBE2I (or UBC9, UBCE9) is a SUMO conjugating enzyme, also called SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I or p18, that attaches SUMO proteins (SUMO1, SUMO2, SUMO3 & SUMO4) to other target proteins, primarily various transcription factors (HIF1A, FOXL2, KAT5, I-kappa-B) with the help of ubiquitin E3 ligases such as CBX4 and RANBP2. UBE2I is also know to interact with a huge array of additional targets as part of its role as a ubiquitin E2 ligase including SALL1, FHIT, RANBP2, Protein inhibitor of activated STAT2, DNMT3A, DNMT3B, Small ubiquitin-related modifier 1, SAE2,RANGAP1,Death associated protein 6, Androgen receptor, ETS1, IPO13, TCF3, TNFRSF1A, DACH1, P53, BLMH, MAP3K1, WT1, TOP1, RAD51, Activating transcription factor 2, and PIAS1. UBE2I expression is largely nuclear and the protein can be found expressed in a wide variety of tissues including heart, skeletal muscle, pancreas, kidney, liver, lung, testis, thymus, placenta and brain. EMD-Millipore’s Anti-UBE2I mouse monoclonal antibody has been tested in western blot on recombinant UBE2I protein as well as HeLa, HepG2 and COS7 cell lysates and in paraffin embedded immunohistochemistry on normal prostate and bladder cancer tissue sections. The antibody has also been successfully tested in fluorescent immunocytochemistry on HepG2 cells in culture and in flow cytometry on HepG2 cells in culture.
Immunogen
Purified recombinant fragment of human UBE2I expressed in E. Coli.
Application
Anti-UBE2I Antibody, clone 1B10 is a highly specific mouse monoclonal antibody, that targets Ubiquitin carrier protein 9 & has been tested in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected UBE2I in prostate and bladder cancer tissues.
Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected UBE2I in HepG2 cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected UBE2I in non serum starved HepG2 cells.
Optimal working dilutions must be determined by end user.
Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected UBE2I in HepG2 cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected UBE2I in non serum starved HepG2 cells.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blotting in HeLa, HepG2, and Cos7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected UBE2I in HeLa, HepG2, and Cos7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected UBE2I in HeLa, HepG2, and Cos7 cell lysates.
Target description
~18 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa, HepG2, and Cos7 cell lysates
HeLa, HepG2, and Cos7 cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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