MABC287

Anti-PXR Antibody, clone 1F10.1

clone 1F10.1, from mouse

• Synonym(s): Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, SXR, PXR
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1F10.1, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), gorilla (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_003880
• UniProt accession no.: O75469
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NR1I2(8856)

Description

General description

Nuclear receptor subfamily 1 group I member 2 (UniProt O75469; also known as Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X nuclear receptor, Steroid and xenobiotic receptor, SXR) is encoded by the NR1I2 (also known as BXR, PAR, PRR, PXR, SAR, SXR, ONR1) gene (Gene ID 8856) in human. Pregnane X receptor (PXR) is a nuclear receptor that displays similarity to PPAR and RXR. PXR is activated by a large number of endogenous and exogenous chemicals including steroids and antibiotics. One of the key roles of PXR is the induction of CYP3A4, a P450 enzyme involved in drug metabolism.

Specificity

Epitope is present in all 7 alternative spliced isoforms (isoform 1A/PRR1-A, 1B/PRR1-B, 1C/PRR1-C, 2A/PRR2-A, 2B/PRR2-V, 2C/PRR2-C, and isoform 3).

Immunogen

KLH-conjugated linear peptide corresponding to human PXR.

Application

Detect PXR using this Anti-PXR Antibody, clone 1F10.1 validated for use in Western Blotting.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PXR in 10 µg of human liver tissue lysate.

Quality

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PXR in 10 µg of human colon tissue lysate.

Target description

~53 kDa observed. Uniprot describes 7 isoforms produced by alternative splicing ranging from ~40 kDa-54 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable