biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
mol wt
calculated mol wt 39.56 kDa
observed mol wt ~27 kDa
species reactivity
mouse
packaging
antibody small pack of 100 μg
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... Clstn3(232370)
Related Categories
General description
Calsyntenin 3beta (UniProt: A0A4P8DYG6; also known as Calsyntenin-3β, CLSTN3β) is encoded by the Clstn3 (also known as Clstn3b) gene (Gene ID: 232270) in murine species. CLSTN3β is a mammal-specific that is preferentially expressed in thermogenic adipocytes and is localized in endoplasmic reticulum. Its expression in brown adipose tissue is about six-fold higher than both the inguinal subcutaneous and the perigonadal visceral white adipose tissue. However, its expression can be induced in the inguinal subcutaneous white adipose tissue upon cold exposure. Brown adipose tissue thermogenesis require innervation by the sympathetic nerves that release norepinephrine, which activates β-adrenergic receptors-cAMP-PKA pathway to drive lipolysis and thermogenic respiration. CLSTN3β is shown to promote S100b protein release that promotes this innervation and its loss is shown to reduce functional sympathetic innervation in adipose tissue. Loss of CLSTN3β also predisposes mice to obesity on a high fat diet. Clstn3β knockout mice exhibit significantly higher fasting glucose levels, impaired glucose tolerance, more lipid accumulation in brown adipose tissue, and liver steatosis. (Ref.: Plucińska, K., et al. (2020). Front. Endocrinol. 11; 579785; Zeng, X., et al. (2019). Nature. 569(7755); 229-235).
Specificity
This rabbit polyclonal antibody detects murine Calsyntenin 3b. It targets an epitope within 18 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of murine Calsyntenin 3b..
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from wild-type brown adipocytes.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Calsyntenin 3b (Clstn3) in lysate from wild-type adipocytes, but not in lysate from Calsyntenin 3b knock out adipocytes.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated CLSTN3b in Immunoprecipitation applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Western Blotting Analysis: A representative lot detected CLSTN3b in Western Blotting applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Immunofluorescence Analysis: 10 ug/mL from a representative lot detected CLSTN3b in mouse primary brown adipocytes (Courtesy of Dr. Xing Zeng @UT Southwestern Medical Center).
Immunofluorescence Analysis: A representative lot detected CLSTN3b in Immunofluorescence applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from wild-type brown adipocytes.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Calsyntenin 3b (Clstn3) in lysate from wild-type adipocytes, but not in lysate from Calsyntenin 3b knock out adipocytes.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated CLSTN3b in Immunoprecipitation applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Western Blotting Analysis: A representative lot detected CLSTN3b in Western Blotting applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Immunofluorescence Analysis: 10 ug/mL from a representative lot detected CLSTN3b in mouse primary brown adipocytes (Courtesy of Dr. Xing Zeng @UT Southwestern Medical Center).
Immunofluorescence Analysis: A representative lot detected CLSTN3b in Immunofluorescence applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CLSTN3b, Cat. No. ABS2236, is a rabbit polyclonal antibody that detects Calsyntenin 3b (Clstn3) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Physical form
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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