DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.
Specificity
Dishevelled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant dishevelled-1 (dvl-1) or dishevelled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.
Immunogen
Synthetic peptide corresponding to amino acids 633-645 of human disheveiled-2 protein.
Application
Research Category Stem Cell Research
Research Sub Category Hematopoietic Stem Cells
Neural Stem Cells
This Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa.
Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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