14-518
PKCε Protein, active, 10 µg
Active, N-terminal His6-tagged, recombinant PKCε, amino acids 2-end. For use in Kinase Assays.
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source
human
Quality Level
recombinant
expressed in Sf21 cells
mol wt
Mw 84.6 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... PRKCE(5581)
General description
N-terminal His6-tagged, recombinant PKCε, amino acids 2-end
PKC Lipid activator (Catalog # 20-133) is required for use.
PKC Lipid activator (Catalog # 20-133) is required for use.
Product Source: Human PKCε expressed in Sf21 insect cells
Biochem/physiol Actions
Protein Target: PKCε
Packaging
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-518M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-518M when ordering the 250μg size.
Quality
Routinely evaluated by phosphorylation of PKC Substrate Peptide
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Guo-Zhong Tao et al.
Biochemical and biophysical research communications, 351(3), 708-712 (2006-11-07)
Phosphorylation and O-GlcNAcylation of keratin 18 (K18) are highly dynamic and involve primarily independent K18 populations. We used in vitro phosphorylation and O-GlcNAcylation of wild-type, phospho-Ser52, glyco-Ser48, and Ser-to-Ala mutant 17mer peptides (K18 amino acids 40-56), which include the major
Thomas B Schaffer et al.
Cell reports, 25(9), 2470-2483 (2018-11-30)
Protein kinase C (PKC)-dependent mechanisms promote synaptic function in the mature brain. However, the roles of PKC signaling during synapse development remain largely unknown. Investigating each brain-enriched PKC isoform in early neuronal development, we show that PKCε acutely and specifically
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