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S4760

Sigma-Aldrich

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C19H25N5O8
CAS Number:
Molecular Weight:
451.43
Beilstein:
2928351
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate

Assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

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General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease, pancreatic elastase and serine endopeptidase.

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate
  • for screening elastase activity in lyophilized earthworm fibrinolytic enzymes
  • for quantifying elastase activity in sheep milk
  • in screening honeybee inhibitors in serine protease inhibition assay

Substrates

Elastase substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of endogenous enzymes in proteolysis of sheep milk
Albenzio M, et al.
Journal of Dairy Science, 92(1), 79-86 (2009)
In Situ and Multisubstrate Detection of Elastase Enzymatic Activity External to Microdialysis Sampling Probes Using LC- ESI-MS
Wang Y, et al.
Analytical Chemistry, 80(6), 2050-2057 (2008)
Nguyen Van Quan et al.
Foods (Basel, Switzerland), 8(12) (2019-11-27)
We previously reported the inhibitory potentials of momilactones A (MA) and B (MB) against key enzymes related to type 2 diabetes and obesity. In this study, antioxidant and anti-skin-aging activities of MA and MB were investigated and compared with tricin
M Ogawa et al.
Research communications in chemical pathology and pharmacology, 55(2), 271-274 (1987-02-01)
A human urinary trypsin inhibitor preparation, MR-20, strongly inhibited granulocyte elastase activity. The concentration of MR-20 causing 50% inhibition of the hydrolysis of Suc(Ala) 3pNA by granulocyte elastase was estimated to be 6.4 X 10(-8) M (11 U/ml).
William R Swindell et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Tetrahexyldecyl Ascorbate (THDC) is an L-ascorbic acid precursor with improved stability and ability to penetrate the epidermis. The stability and transdermal penetration of THDC, however, may be compromised by the oxidant-rich environment of human skin. In this study, we show

Articles

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Protocols

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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