R1905

Anti-RACK1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-GNB2L1, Anti-Gnb2-rs1, Anti-Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1, Anti-H12.3, Anti-HLC-7, Anti-Lung cancer oncogene 7, Anti-PIG21, Anti-Proliferation-inducing gene 21, Anti-Receptor of Activated Protein Kinase C1, Anti-protein homologous to chicken B complex protein
• MDL number: MFCD01634078
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~30 kDa
• species reactivity: human, rat, mouse
• technique(s): immunoprecipitation (IP): 5-10 μL using HEK-293T cell lysates; indirect immunofluorescence: 1:100-1:200 using paraformaldehyde-fixed HeLa cells; western blot: 1:500-1:1,000 using HEK-293T cell lystes
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GNB2L1(10399) ; mouse ... Gnb2l1(14694) ; rat ... Gnb2l1(83427)

Description

General description

Receptors for activated C-kinase (RACKs) are scaffold protein for Protein kinase C (PKC). It is composed of seven WD (Trp-Asp) repeats, thus resembling the structure of the subunit of G proteins.

Specificity

Anti-RACK1 (N-terminal) is produced in rabbit and specifically recognizes human RACK1. Anti-RACK1 (N-terminal) antibody is specific for human RACK1 (approx. 30 kDa). The antibody also reacts with rat and mouse RACK1. In immunoblotting, staining of the RACK1 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 2-16 of human RACK1, conjugated to KLH via an C-terminal added cysteine residue. The corresponding sequence is conserved in human, rat and mouse.

Application

Anti-RACK1 (N-terminal) antibody is suitable for use in indirect immunofluorescence (1:100-1:200 using paraformaldehyde-fixed HeLa cells), western blot (1:500-1:1,000 using HEK-293T cell lysates) and immunoprecipitation (5-10 μL using HEK-293T cell lysates).

It is also used in immunoblotting.

Biochem/physiol Actions

RACK1 is a receptor for activated C kinases that functions downstream of the growth factor receptor signaling pathway. RACK1 also modulates several functions such as cell adhesion, chemotaxis and protrusion. Increased RACK1 expression has been associated with cell migration and metastasis in breast carcinoma. Thus, RACK1 can be used as a prognostic indicator for breast cancer

Receptors for activated C-kinase (RACKs) anchor activated Protein kinase C (PKC) at the site of translocation. Receptors for activated C-kinase 1 (RACK1) is the selective RACK for PKC-βII. RACK1 anchors other signaling enzymes such as Sarcoma (Src) tyrosine kinase, integrin and phosphodiesterase 4D5. The ability of RACK1 to interact simultaneously with different signaling molecules, allows it to integrate inputs from distinct signaling pathways. Accumulating evidence attributes to it the role of regulating several major nervous system pathways. RACK1 is a ribosomal protein and can make direct contact with ribosomal RNA. It recruits activated PKC to the ribosome, which in turn, phosphorylates initiation factor 6, leading to ribosome assembly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves