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PRS2265

Sigma-Aldrich

Anti-Nephrin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

CNF, NPHN, Renal glomerulus-specific cell adhesion receptor

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human, mouse

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

NCBI accession no.

NP_004637

UniProt accession no.

O60500

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NPHS1(4868)

General description

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. It is a 1241-residue transmembrane protein. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomalrecessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

Immunogen

Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human
Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.

Linkage

The action of this antibody can be blocked using blocking peptide SBP2265.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
92172004
22650604
2265-0604

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Ling Xie et al.
Drug design, development and therapy, 14, 527-538 (2020-02-28)
Epithelial-mesenchymal transition (EMT) may contribute to podocyte dysfunction in diabetic nephropathy (DN). Aiming to identify novel therapeutic options, we investigated the protective effects of Panax notoginseng (PN) on podocyte EMT in diabetic rats and explored its mechanisms. Diabetes was induced
Vladislav E Sobolev et al.
International journal of molecular sciences, 22(24) (2021-12-25)
The delayed effects of acute intoxication by organophosphates (OPs) are poorly understood, and the various experimental animal models often do not take into account species characteristics. The principal biochemical feature of rodents is the presence of carboxylesterase in blood plasma
Positionally Cloned Gene for a Novel Glomerular Protein?Nephrin?Is Mutated in Congenital Nephrotic Syndrome
Marjo Kestilla
Molecular Cell, 1(4), 575-582 (1998)
Balajikarthick Subramanian et al.
Kidney international, 90(2), 363-372 (2016-06-29)
Mutations in the INF2 (inverted formin 2) gene, encoding a diaphanous formin family protein that regulates actin cytoskeleton dynamics, cause human focal segmental glomerulosclerosis (FSGS). INF2 interacts directly with certain other mammalian diaphanous formin proteins (mDia) that function as RhoA
Dong Wang et al.
International journal of molecular sciences, 19(1) (2018-01-06)
Obesity and obesity related kidney and liver disease have become more prevalent over the past few decades, especially in the western world. Sodium-glucose cotransporter 2 (SGLT2) inhibitors are a new class of antidiabetic agents with promising effects on cardiovascular and
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Questions

1–2 of 2 Questions  

  1. Why does the Nephrin from mouse kidney indicate a molecular weight of a little less than 100 kDa on the Western blot shown on the webpage for Product PRS2265, anti-Nephrin antibody produced in rabbit?

    1 answer

    1. Based on the literature, one would expect the Nephrin to show up at a higher molecular weight. Unfortunately, we do not have a good explanation for the mouse Nephrin band showing up at a molecular weight of a little less than 100 kDa. It might be due to a cleavage event, but we have not read of that happening with Nephrin. The antibody may be detecting an alternative splicing product, but again, we have not seen anything like that mentioned in the literature on Nephrin.The sequence used as the antigen for this antibody does not possess homology to any other protein. And the PRS2265 antibody is quite specific for this sequence, based on the results when using a blocking peptide as shown in lane B of the blot: Supplier is confident that the antibody is detecting Nephrin but is not able to be of more help in explaining the lower-than-expected molecular weight.According to the Uniprot link for mouse nephrin, the mature form of mouse Nephrin has been described as a protein that is a single polypeptide of 1221 amino acids (1256 minus the signal peptide of 35 amino acids)So based on the amino acids alone, one would expect a band close to 130 kDa.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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