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D3447

Sigma-Aldrich

Doxycycline hydrochloride

Synonym(s):

2-Naphthacenecarboxamide, 4-(dimethylamino)- 1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, hydrochloride (1:1), (4S,4aR,5S,5aR,6R,12aS)-

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About This Item

Empirical Formula (Hill Notation):
C22H24N2O8 · HCl
CAS Number:
10592-13-9
Molecular Weight:
480.90
MDL number:
MFCD23136018
UNSPSC Code:
51284028
NACRES:
NA.85
Pricing and availability is not currently available.

biological source

synthetic

Quality Level

200

form

powder

color

yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycoplasma
parasites

Mode of action

protein synthesis | interferes

storage temp.

−20°C

InChI

1S/C22H24N2O8.ClH/c1-7-8-5-4-6-9(25)11(8)16(26)12-10(7)17(27)14-15(24(2)3)18(28)13(21(23)31)20(30)22(14,32)19(12)29;/h4-7,10,14-15,17,25-27,31-32H,23H2,1-3H3;1H/b21-13-;/t7-,10+,14+,15-,17-,22-;/m0./s1

InChI key

VLUQVUWDECWBTL-UQVCFKGQSA-N

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General description

Doxycycline is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is a Broad spectrum antibiotic and bacteriostatic. Doxycycline shows antiprotozoal properties and is a potent inhibitor of MMPs (matrix metalloproteinases) in vivo.

Application

Doxycycline hydrochloride has been used for the induction of shRNA expression and induction of expression vectors.[1][2][3]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Keep container tightly closed in a dry and well-ventilated place.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302,H361d

Hazard Classifications

Acute Tox. 4 Oral - Repr. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000401303
0000401303
0000348652
0000261824
0000222657

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David van Dijk et al.
Cell, 174(3), 716-729 (2018-07-03)
Single-cell RNA sequencing technologies suffer from many sources of technical noise, including under-sampling of mRNA molecules, often termed "dropout," which can severely obscure important gene-gene relationships. To address this, we developed MAGIC (Markov affinity-based graph imputation of cells), a method that
John D Chan et al.
Nature communications, 8(1), 1910-1910 (2017-12-07)
Schistosomiasis is a debilitating tropical disease caused by infection with parasitic blood flukes. Approximately 260 million people are infected worldwide, underscoring the clinical and socioeconomic impact of this chronic infection. Schistosomiasis is treated with the drug praziquantel (PZQ), which has
Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress
Beate H et al.
BMC Cancer (2018)
C3 transferase gene therapy for continuous conditional RhoA inhibition
Claire-Anne G et al.
Neuroscience, 339 (2016)
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells
Debra A et al.
Molecular Vision, 22(40) (2016)
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. IS DOXYCYCLINE HYDROCHLORIDE (D3447) SOLUBLE IN WATER 50 MG/ML?

    1 answer

    1. This product is soluble in water at 50 mg/ml.

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  4. What is the best way to solubilize the product for cell culture and animal use?

    1 answer

    1. This product has not been tested for use in cell culture. However, product D5207 is a cell culture tested Doxycycline Hyclate. The Hyclate and the Hydrochloride salt forms are soluble in water at roughly equal rates. For dosing purposes, the unique molecular weight of each must be considered. Please see the link below to the product data sheet for the cell culture version of this product. This information offered in this document may be applied to the D3447:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/402/297/d5207dat.pdf

      Note that neither of these products are sterile. Prepared stock solutions must be filter sterilized prior to adding them to the culture medium.

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