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CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

Synonym(s):

Acid Phosphatase Enzyme Activity Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
42010102
NACRES:
NA.84

Quality Level

usage

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Biochem/physiol Actions

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Kit Components Only

Product No.
Description

  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets

  • Citrate buffer solution, 0.09 M pH 4.8 100 mL

  • p-Nitrophenol Standard solution 10 mM 1 mL

  • Acid phosphatase control enzyme .2 mL

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Archana Vaithilingam et al.
BMC cell biology, 14, 35-35 (2013-08-14)
Endolysosomes play a key role in maintaining the homeostasis of the cell. They are made of a complex set of proteins that degrade lipids, proteins and sugars. Studies involving endolysosome contribution to cellular functions such as MHC class I and
Stephen R Hennigar et al.
The Journal of nutrition, 145(9), 1999-2005 (2015-07-24)
Lactation failure is common in overweight and obese women; however, the precise mechanism remains unknown. We tested the hypothesis that obesity-induced inflammation in the mammary gland (MG) redistributes subcellular zinc pools to promote cell death of mammary epithelial cells (MECs)
Lan Xiao et al.
OncoTargets and therapy, 9, 1105-1114 (2016-04-14)
To identify the role of YAP in cisplatin resistance in human ovarian cancer cells and in the regulation of autophagy in these cancer cells. The cisplatin-sensitive OV2008 parental cell line and its cisplatin-resistant variant C13K were cultured. RNA interference was
Fan Zhang et al.
American journal of physiology. Cell physiology, 301(2), C421-C430 (2011-05-27)
It is well known that the mutation of TRP-ML1 (transient receptor potential-mucolipin-1) causes mucolipidosis IV, a lysosomal storage disease. Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed
Messika Revel et al.
Marine pollution bulletin, 150, 110627-110627 (2019-10-28)
The aim of the present study was to evaluate the presence and potential toxic effects of plastic fragments (<400 μm) of polyethylene and polypropylene on the Pacific oyster Crassostrea gigas. Oysters were exposed to environmentally relevant concentrations (0, 0.008, 10, 100 μg

Articles

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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