C2488
Citrate Buffer Solution, 0.09 M
Synonym(s):
Citric Acid Buffer Solution
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About This Item
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
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description
Contains 1% chloroform
pH 4.8 at 25 °C.
Quality Level
form
solution
concentration
0.09 M
pH
4.8 (25 °C)
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Related Categories
General description
Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues.[1] In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis.[2] With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.
Application
Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments.[3][4] It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.[5]
Other Notes
Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 2 - STOT RE 2 Oral
Target Organs
Liver,Kidney
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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