B8041
Biglycan from bovine articular cartilage
essentially salt-free, lyophilized powder
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About This Item
biological source
bovine articular cartilage
Quality Level
form
essentially salt-free, lyophilized powder
mol wt
200-350 kDa
packaging
glass bottle of 0.5 mg
technique(s)
cell culture | mammalian: suitable
solubility
water: soluble ≥2.00 mg/mL, clear, colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
cow ... BGN(280733)
General description
proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains
Biochem/physiol Actions
Biglycan interacts with collagen type I and II, fibronectin and TGF-ß. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding.
Components
Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains.
Caution
Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.
Preparation Note
This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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