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Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade

Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade
1 of 1 reviewers received a sample product or took part in a promotion

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

Synonym(s):

Organic Solvent Mix for DNA/RNA

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.21
grade:
Molecular Biology
Assay:
≥99% phenol basis (titration)
>99% chloroform basis (HPLC)
Pricing and availability is not currently available.

grade

Molecular Biology

Quality Level

Assay

≥99% phenol basis (titration)
>99% chloroform basis (HPLC)

form

liquid (Two-layer)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

colorless

shipped in

ambient

storage temp.

2-8°C

General description

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.

Warning

Toxicity: Toxic (F)

Reconstitution

Following equilibration, refrigerate (4°C).

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuyan You et al.
PloS one, 14(9), e0222292-e0222292 (2019-09-26)
The giant panda (Ailuropoda melanoleuca) is a native species to China. They are rare and endangered and are regarded as the 'national treasure' and 'living fossil' in China. For the time being, there are only about 2500 giant pandas in

Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

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  1. Oman
    • Review 1
    • Votes 0
    5 out of 5 stars.

    excellent presentation for each products but the back order items not assure

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