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1.25085

Millipore

Fractogel® EMD TMAE Hicap (M)

RoboColumn Fractogel® TMAE Hicap (M), volume 0.2 mL, prepacked column

Synonym(s):

RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml

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About This Item

UNSPSC Code:
41115711

ligand

(Trimethylammoniumethyl)

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~180 cm/hr max. flow rate

column I.D. × L

5 mm × 10 mm

volume

0.2 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~180 mg, BSA binding capacity

application(s)

mAb
recombinant protein
vaccine development

separation technique

anion exchange

storage temp.

room temp

General description

5 x 10 mm

Features and Benefits

RoboColumn Fractogel® TMAE Hicap (M), 0.2 ml enables users to:

  • Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
  • Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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