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T9028

Sigma-Aldrich

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

clone TUB-1A2, ascites fluid

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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About This Item

MDL number:
MFCD00164753
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TUB-1A2, monoclonal

contains

15 mM sodium azide

species reactivity

human, plant, animal

technique(s)

indirect immunofluorescence: 1:800 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

isotype

IgG3

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA4A(7277) , TUBB(203068)

General description

Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.
The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Specificity

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.

Immunogen

peptide containing the carboxy-terminal amino acids of α-tubulin

Application

Monoclonal Anti-Tyrosine Tubulin has been used in:
  • indirect immunofluorescent labelling
  • immunoblotting technique
  • immunocytochemical staining

Biochem/physiol Actions

Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Malkolm Graffe et al.
The Journal of general physiology, 146(1), 109-117 (2015-07-01)
A set of bipolar cells in the retina of goldfish contains giant synaptic terminals that can be over 10 µm in diameter. Hundreds of thousands of synaptic vesicles fill these terminals and engage in continuous rounds of exocytosis. How the
Microtubule Structures
Molecular Cell Biology. 4th edition. (2000)
Nagayasu Nakanishi et al.
BMC biology, 12, 26-26 (2014-04-01)
The evolutionary origin of gastrulation--defined as a morphogenetic event that leads to the establishment of germ layers--remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite
L J Lee et al.
Journal of cellular biochemistry, 81(1), 162-171 (2001-02-17)
Conflicting data for the effects of colchicine on cholesterol transport and steroidogenesis raise the question of the role of microtubules in cholesterol transport from the lipid droplet to mitochondria in steroidogenic cells. In this study, using corticosterone radioimmunoassay and immunofluorescence
Silvia Sintoni et al.
Neural development, 7, 1-1 (2012-01-10)
In the decapod crustacean brain, neurogenesis persists throughout the animal's life. After embryogenesis, the central olfactory pathway integrates newborn olfactory local and projection interneurons that replace old neurons or expand the existing population. In crayfish, these neurons are the descendants
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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