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S8820

Sigma-Aldrich

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

Synonym(s):

Protease Inhibitor Tablets, protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

biological source

bovine lung
bovine milk

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

Related Categories

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:
  • to wash raw semen for membrane protein analysis
  • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
  • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
  • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Features and Benefits

  • Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
  • Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
  • Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
  • Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tran Hong Nha Nguyen et al.
Molecular & cellular proteomics : MCP, 11(11), 1140-1155 (2012-07-31)
Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia
Nihar J Mehta et al.
Free radical biology & medicine, 101, 44-52 (2016-11-05)
The dopamine oxidation product cysteinyl-dopamine has attracted attention as a contributor to the death of dopaminergic neurons in Parkinson's disease. Treatment of cysteinyl-dopamine with hypochlorite yields an even more cytotoxic product. This product has potent redox-cycling activity and initiates production
Immunometabolic profiling of T cells from patients with relapsing-remitting multiple sclerosis reveals an impairment in glycolysis and mitochondrial respiration
La Rocca C, et al.
Journal of biological and chemical chronicles, 77, 39-46 (2017)
Heather M Brechbuhl et al.
The American journal of pathology, 179(1), 367-379 (2011-06-28)
The purpose of this study was to determine whether β-catenin regulates basal cell fate determination in the mouse trachea. Analysis of TOPGal transgene reporter activity and Wnt/β-catenin pathway gene expression suggested a role for β-catenin in basal cell proliferation and
Giedrius Steponaitis et al.
Oncology letters, 12(5), 3305-3311 (2016-12-03)
Astrocytomas are one of the most common brain tumours; however, the current methods used to characterize these tumours are inadequate. The establishment of molecular markers may identify variables required to improve tumour characterization and subtyping, and may aid to specify
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Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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